Keragaman genetik cendawan entomopatogen Metarhizium anisopliae (Metsch.) berasal dari tanah pertanaman kelapa sawit berdasarkan penanda RAPD

نویسندگان

چکیده

Metarizhium anisopliae (Metsch.) is an entomopathogenic fungus that attacks Oryctes rhinoceros (Linnaeus). This one of the potential bioagents environmentally friendly. The purpose this study was to isolate M. from various oil palm plantation soil samples and see its genetic diversity based on RAPD markers. method used in research morphological molecular identification (ITS), testing using Fifteen isolates isolated 15 sites North Sumatra were examined for their diversity. On basis characteristics, all identified as anisopliae. result sequencing with ITS primer showed had a 85–99% homology brunneum reference strains gen bank database National Center Biotechnology Information (NCBI). Eight primers generated 39 scorable bands which 38 (98,44%) them polymorphic. Clustering analysis performed profiles Neighbour-Joining Tree formed 3 groups. Key words: fungi, diversity, sequencing,

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Efficacy of Entomopathogenic Fungi, Paecilomyces fumosoroseus, Beauvena bassiana and Metarhizium anisopliae var. majus Against Crocidolomia binotalis (Lepidoptera; Pyralidae)

Bioasai di makmal tiga pencilan tempatan entomopatogen hyphomycete ke atas ulat hati kubis, Crocidolomia binotalis Zeller (Lepidoptera: Pyralidae) dan efikasi lapangan beberapa persediaan formulasi Paecilomyces fumosoroseus (Wise) Brown & Smith telah dinilai. Bioasai dos-kernatian menunjukkan kesemua pencilan berupaya menyebabkan maut ke atas larva instar kedua. Kebanyakan larva menjadi moribun...

متن کامل

Viability of Metarhizium anisopliae conidia (metsch.) Sorok preserved in packages containing silica gel

Background The entomopathogenic fungi are important microbial agents for their efficient performance as microbial biopesticides in pest control. The development of commercial products formulated with entomopathogenic fungi is in a promising market for biopesticides. However, the fungi can lose their viability when exposed to unfavorable conditions of temperature, humidity and ultraviolet radiat...

متن کامل

Land Dispute Resolution in the Political Reform at the time of Decentralization in Indonesia

Tulisan ini mengkaji dampak dari tahun-tahun awal reformasi politik, demokratisasi, dan otonomi daerah terhadap masalah agraria. Selama masa Orde Baru, masyarakat setempat tidak dilibatkan dalam hal kepemilikan/pengelolaan sumber daya tanah. Sengketa tanah berkembang menjadi isu politik di banyak daerah di Indonesia. Data yang disajikan dalam tulisan ini berasal dari Sumatra Selatan, salah satu...

متن کامل

Fungal keratitis caused by Metarhizium anisopliae complex

PURPOSE To report a case of fungal keratitis caused by Metarhizium anisopliae complex. METHODS Case report. RESULTS Our patient presented with a central corneal infiltrate. Fungal culture yielded a Metarhizium species. She was started on antifungal agents with no significant improvement. A therapeutic corneal transplant was performed after perforation. At two years follow up, she was free o...

متن کامل

[Variability in esterases of Metarhizium anisopliae].

The variability in esterases of the entomogenous fungus Metarhizium anisopliae was determined electrophoretically on 8.5% polyacrylamide gel. Ten isolates from diverse taxonomic groups of insects were analyzed. The electrophoretic analysis showed differences and similarities between these isolates and it was possible to distinguish six different patterns. The results obtained show a great polym...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Jurnal Entomologi Indonesia

سال: 2023

ISSN: ['1829-7722', '2089-0257']

DOI: https://doi.org/10.5994/jei.20.1.22