Keragaman genetik cendawan entomopatogen Metarhizium anisopliae (Metsch.) berasal dari tanah pertanaman kelapa sawit berdasarkan penanda RAPD
نویسندگان
چکیده
Metarizhium anisopliae (Metsch.) is an entomopathogenic fungus that attacks Oryctes rhinoceros (Linnaeus). This one of the potential bioagents environmentally friendly. The purpose this study was to isolate M. from various oil palm plantation soil samples and see its genetic diversity based on RAPD markers. method used in research morphological molecular identification (ITS), testing using Fifteen isolates isolated 15 sites North Sumatra were examined for their diversity. On basis characteristics, all identified as anisopliae. result sequencing with ITS primer showed had a 85–99% homology brunneum reference strains gen bank database National Center Biotechnology Information (NCBI). Eight primers generated 39 scorable bands which 38 (98,44%) them polymorphic. Clustering analysis performed profiles Neighbour-Joining Tree formed 3 groups. Key words: fungi, diversity, sequencing,
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ژورنال
عنوان ژورنال: Jurnal Entomologi Indonesia
سال: 2023
ISSN: ['1829-7722', '2089-0257']
DOI: https://doi.org/10.5994/jei.20.1.22